Biological Buffers Tris CAS NO 77-86-1

Tris hydroxymethyl aminomethane Biological Buffers CAS NO 77-86-1 99%
 
 
Quick Detail:
 

 
 
Description:
>  Formula:: C4H11NO3
>  Weight:: 121.135
>  Appearance: White crystalline powder
>  MP/BP:168-171℃
>  Storage: room temperature
>  HS:2922199090
>  Safety Description: S26 S36
>  Danger Description: R36/37/38
>  Danger Symbol: Xi
 
 
Applications:  

1.  A. Tris is a commonly used buffer in biological laboratory, such as DNA Extraction.
    B. Tris is also used as a primary standard to standardize acid solutions for chemical       analysis.
    C. Tris is used to increase membrane permeability of cell membranes.
2.  Location
    A. biology/biochemistry laboratory, pharmaceutical factory, cosmetics factory

    3. Note:
      Tris buffer is temperature sensitive and should be used at the temperature at which it was originally

      pH to avoid inaccuracy, in addition, Tris inhibits a number of enzymes, and therefore it should be

      used  with care when studying proteins.
 

 
Specifications

Synonyms:

1,1,1-tris(hydroxymethyl)-methanamin;1,1,1-tris(hydroxymethyl)-methylamin;1,1,1-tris(hydroxymethyl)methylamine;1,3-Propanediol,2-amino-2-(hydroxymethyl)-;2-amino-2-(hydroxymethyl)-3-propanediol;2-Amino-2-hydroxymethylpropanediol;2-Amino-2-methylol-1,3-propanediol;3-Propanediol,2-amino-2-(hydroxymethyl)-1;2-Amino-2-hydroxymethyl-1,3-propanediol;

TRIS; Tromethamine; Tromethane; tris(hydroxyme.)aminomethane; trizma base; Trometamol; tri(hydroxymethyl)methylamine; Tris(hydroxymethyl) methylamine Tris buffer 99.8+ %;

Trihydroxymethyl Aminomethane; Tris, Ultra Pure; Tris, Tissue Culture Tested Tris(hydroxymethyl)aminomethane, Tissue Culture Tested; Tris, Alcohol Free Tris(hydroxymethyl)aminomethane, Alcohol Free; Tris(hydroxymethyl)aminomethane, Ultra Pure, Molecular Biology Grade; Tris EDTA acetate buffer; THAM; Trimethanolaminomethane; Tri(hydroxymethyl)aminomethane; Tris(hydroxymethyl)aminomethane;

2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris hydroxyl methyl amino methane;

2-amino-2-(hydroxymethyl)propane-1,3-diol; 1,3-dihydroxy-2-(hydroxymethyl)propan-2-aminium; (methylamino)methanetriol; Tris(hydroxymethyl) aminomethane

Related categories:

Biochemistry;Reagents for Electrophoresis;Buffer;ACS Grade Buffers;Amino Alcohols;Biological Buffers;Buffers A to Z;Building Blocks;Chemical Synthesis;Organic Building Blocks;Oxygen Compounds

Related Products:56-40-6;10043-35-3;67-68-5;3483-12-3;1185-53-1;9002-93-1

The determination of PH

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, 

determination of the pH used have been calibrated pH meter.

The determination of solubility

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve,

 observe the solution.

Determination of melting point

   Instrument:Micro melting point apparatus WRS-2A, Melting point tubes

  Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample 

was compressed into about 1cm high .When the actual temperature reaches the set temperature and 

stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.

The determination of KF

Use MA-1 intelligent Carle Fischer moisture tester to measure water content

Determination of iron content

  Preparation of standard iron solution

  Weigh ammonium iron(III) sulfate [FeNH4(SO4)2·12H2O] 0.863g .Placed in 1000mL volumetric flask, 

add water to dissolveand add sulfuric acid 2.5mL.Diluted with water to the mark, shake, as the stock 

solution.Before use, exact amount of stock solution 1mL, placed in 100mL flask, diluted with water to 

the mark and shake.

  Weigh 1g sample, placed in a crucible.Adding sodium carbonate 2g and mixed.Burning at 740 ℃,

the residue is dissolved in dilute hydrochloric acid 15ml , displacing 50mL Nessler colorimetric tube ,

add dilute hydrochloric acid 4mL and ammonium persulfate 50mg , Diluted with water to make into 35mL, add 30% ammonium thiocyanate solution 3mL, plus the amount of water diluted into 50mL, shaked. 

If the color, take 1mL standard iron solution into 50mL Nessler colorimetric tube, add water to 25mL,4mL 

dilute hydrochloric acid and 50mg ammonium persulfate , diluted with water to 35mL, then plus 30% 

ammonium thiocyanate solution 3mL, plus the amount of water diluted into 50mL, shake comparisons.

Detection of heavy metals

     The meaning of heavy metals of this act is under the specified experimental condition, which can 

coloration effects with sodium sulfide or thioacetamide  .The glasses of preparation or storage is not 

allowed to contain lead.

5.8.1  Reagents

  a) Lead dinitrate:1μg/mL

   b) Sodium hydroxide :10%(m/v)

   c) Sodium sulfide :10%(m/v)

5.8.2  Preparation of lead standard solution

     Weigh lead dinitrate  0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water

 50mL, diluted with water to the mark , shake, as the stock solution.

5.8.3 Determination of step

   Before use, the exact amount of stock solution 1mL, relocated to a 100ml volumetric flask, dilute with

 water to the mark, shake, limit the use of the day .

   Weigh sample 1g, after adding sodium hydroxide solution 5mL and 20mL of water to dissolve, set

 Nessler colorimetric tube, add 5 drops of sodium sulfide , shake , compared the color with 5mL lead

 standard solution after the same treatment,  not deeper

The determination of UV absorbance

  A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask, 

after constant volume with pure water, at a specific wavelength by 2cm cuvette was measured by 

UV spectrophotometer.